Product Name	Rabbit Anti-BHMT (p-T45) Polyclonal Antibody
Cat No.	SLA0135
Chinese Name	磷酸化BHMT抗体
Alias	BHMT1_RAT; Bhmt; 2.1.1.5; BHMT1_HUMAN; BHMT1; HEL-S-61p; betaine--homocysteine S-methyltransferase; betaine homocysteine methyltransferase; phi-crystallin; BHMT | BHMT1 (phospho-T45); p-BHMT1; phospho-BHMT1; p-BHMT; BHMT | BHMT1 (phospho-Thr45)
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human,Mouse,Rat
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human BHMT1 around the phosphorylation site of Thr45
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	45 kDa
Subcellular Location	Cytoplasm
Gene ID	635
Swiss-Prot	Q93088
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (SLA0135) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRPand DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (SLA0135) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRPand DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (SLA0135) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRPand DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (SLA0135) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRPand DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (SLA0135) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRPand DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.