Product Name	Recombinant Rabbit Anti-ERK1&ERK2 (p-T202/Y204/T185/Y187) Monoclonal Antibody
Cat No.	SLA3101M
Chinese Name	磷酸化丝裂原活化蛋白激酶1/2重组兔单抗
Alias	MK03_HUMAN; MAPK3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1 (ERK-1); Insulin-stimulated MAP2 kinase; MAP kinase isoform p44 (p44-MAPK); Microtubule-associated protein 2 kinase; p44-ERK1; 2.7.11.24; ERK1; PRKM3; MK01_HUMAN; MAPK1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2 (ERK-2); MAP kinase isoform p42 (p42-MAPK); Mitogen-activated protein kinase 2 (MAP kinase 2 | MAPK 2); ERK2; PRKM1; PRKM2; ERK; ERK-2; MAPK2; NS13; P42MAPK; p38; p40; p41; p41mapk; p42-MAPK; ERK-1; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; p44-MAPK; mitogen-activated protein kinase 3; ERK1 & ERK2 (phospho-T202/Y204/T183/Y185); p-ERK1 & ERK2; phospho-ERK1 & ERK2; ERK1 & ERK2 (phospho-Thr202/Tyr204/Thr183/Tyr185); p44/42
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5A12
React Species	Human,Rat
Applications	WB (1:500-2000) Flow-Cyt (1:100-200) ICC/IF (1:100-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human ERK1 around the phosphorylation site of T202/Y204
Purification	Protein A
Concentration	1mg/ml
Predicted MW.
Observed MW.	42/44 kDa
Subcellular Location	Nucleus
Gene ID	5594
Swiss-Prot	P27361
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) monoclonal antibody, unconjugated (SLA3101M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. The Hela (treated with 200 ng/ml PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) antibody (SLA3101M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 3. The Jurkat (treated with 200 ng/ml PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) antibody (SLA3101M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 4. The C6 (treated with 200 ng/ml PMA for 30 minutes) (Rat) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) antibody (SLA3101M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 5. 4% Paraformaldehyde-fixed HeLa (treated with 200 ng/ml TPA/PMA for 30 minutes) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187)) monoclonal Antibody, unconjugated (SLA3101M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.