Product Name	Rabbit Anti-RNH1 Polyclonal Antibody
Cat No.	SLA0214
Chinese Name	核糖核酸酶抑制因子1抗体
Alias	IIAE12; RAI; RNH; RINI_HUMAN; RNH1; Placental ribonuclease inhibitor (Placental RNase inhibitor); Ribonuclease/angiogenin inhibitor 1 (RAI); PRI; RINI_MOUSE; Ribonuclease/angiogenin inhibitor 1; RINI_RAT; ribonuclease/angiogenin inhibitor
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Clone No.
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1μg/Test) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human Ribonuclease Inhibitor
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	50 kDa
Observed MW.	48 kDa
Subcellular Location	N/A
Gene ID	6050
Swiss-Prot	P13489
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play role in redox homeostasis.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Ribonuclease Inhibitor polyclonal antibody, unconjugated (SLA0214) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ribonuclease Inhibitor Polyclonal Antibody, Unconjugated (SLA0214) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ribonuclease Inhibitor Polyclonal Antibody, Unconjugated (SLA0214) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ribonuclease Inhibitor Polyclonal Antibody, Unconjugated (SLA0214) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ribonuclease Inhibitor Polyclonal Antibody, Unconjugated (SLA0214) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Ribonuclease Inhibitor antibody (SLA0214): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (SL60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.