Product Name	Recombinant Rabbit Anti-PKM Monoclonal Antibody
Cat No.	SLA1401M
Chinese Name	丙酮酸激酶M2重组兔单抗
Alias	CTHBP; HEL-S-30; OIP3; PK3; PKM2; TCB; THBP1; p58; Pk-2; Pk-3; PK; PKM12; KPYM_HUMAN; PKM; Cytosolic thyroid hormone-binding protein (CTHBP); Opa-interacting protein 3 (OIP-3); Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1 (THBP1); Tumor M2-PK; Tyrosine-protein kinase PKM2; 2.7.1.40; PK2; KPYM_MOUSE; Pykm; KPYM_RAT; pyruvate kinase M1/2; pyruvate kinase, muscle
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	9H2
React Species	Human,Mouse,Rat
Applications	WB (1:1000-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PKM
Purification	affinity purified by Protein A
Predicted MW.	58 kDa
Observed MW.	60 kDa
Subcellular Location	Nucleus
Gene ID	5315
Swiss-Prot	P14618
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PKM2 monoclonal antibody, unconjugated (SLA1401M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PKM2 Monoclonal Antibody, Unconjugated(SLA1401M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PKM2 Monoclonal Antibody, Unconjugated(SLA1401M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PKM2 Monoclonal Antibody, Unconjugated(SLA1401M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PKM2 Monoclonal Antibody, Unconjugated(SLA1401M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining	Figure 6. The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PKM2 antibody (SLA1401M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.