Product Name	Recombinant Rabbit Anti-SPP1 Monoclonal Antibody
Cat No.	SLA0601M
Chinese Name	骨桥蛋白/分泌型磷蛋白1重组兔单抗
Alias	BNSP; BSPI; ETA-1; OPN; 2AR; Apl-1; Bsp; Eta; OP; Opnl; Ric; Spp-1; OSP; OSTP_HUMAN; SPP1; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1 (SPP-1); Urinary stone protein; Uropontin; OSTP_MOUSE; Calcium oxalate crystal growth inhibitor protein; Early T-lymphocyte activation 1 protein; Minopontin; OSTP_RAT; 2b7; secreted phosphoprotein 1; osteopontin; bone sialoprotein I; early T-lymphocyte activation 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	1D1
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human Osteopontin protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	35 kDa
Observed MW.	63 kDa
Subcellular Location	Secreted
Gene ID	6696
Swiss-Prot	P10451
Storage	Size : 25ul/50ul/100ul/200ul 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Size : 200ug (PBS only) 0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Recombinant human Osteopontin protein, C-His (HEK293)(SL43600P) Primary: Anti-Osteopontin (SLA0601M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 63 kDa	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated(SLA0601M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated(SLA0601M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated(SLA0601M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated(SLA0601M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated(SLA0601M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated(SLA0601M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

• Minimum order: 1 unit
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• Price: USD, per unit (25ul / 50ul / 100ul / 200ul / 200ug (PBS only))