Product Name	Recombinant Rabbit Anti-AZU1 Monoclonal Antibody
Cat No.	SLA0515M
Chinese Name	天青素/肝素结合蛋白单克隆抗体
Alias	AZAMP; AZU; CAP37; HBP; HUMAZUR; NAZC; hHBP; CAP7_HUMAN; AZU1; Cationic antimicrobial protein CAP37; Heparin-binding protein (HBP | hHBP); azurocidin 1; cationic antimicrobial protein 37; heparin-binding protein; neutrophil azurocidin
Host Species	Mouse
Isotype	IgG1
Clonality	Monoclonal
Clone No.	3G8
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant Human Azurocidin Protein
Purification	affinity purified by Protein A
Predicted MW.	24 kDa
Observed MW.	38-42 kDa
Subcellular Location	Cytoplasm
Gene ID	566
Swiss-Prot	P20160
Storage	0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1:Recombinant Human Azurocidin Protein at 100ng Primary: Mouse Anti-Azurocidin Monoclonal Antibody at 1/1000 dilution (Cat.SLA0515M) Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 28.6kD Observed band size: 38-42kD	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Acute choriitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (SLA0515M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (SLA0515M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (SLA0515M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (SLA0515M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (SLA0515M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 7. Measured by its binding ability in a indirect ELISA. Immobilized Human Azurocidin, His Tag (Cat. SL43022P) at 2 μg/mL (100 μL/well) can bind Anti-HumanAzurocidin monoclonal antibody, the minimum detection concentration is 4ng/mL

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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• Price: USD, per unit (100ug)