DESCRIPTION

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles. Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrincontaining complexes, mediating cell migration, adhesion and integrin signalling. Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	20	20.68	1.44	6.97
2	20	4.45	0.28	6.20
3	20	1.04	0.09	8.87

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	24	18.42	1.39	7.54
2	24	4.37	0.23	5.17
3	24	1.12	0.07	6.38

RECOVERY

The recovery of human Talin-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	82	72-87
	1:4	80	71-89
Cell lysate	1:64	84	78-89
	1:128	89	80-102

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:8.)

		Human serum	Cell lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	99	105
	Range (%)	91-107	101-108
1:8	Average% of Expected	88	103
	Range (%)	84-92	89-118
1:16	Average% of Expected	-	83
	Range (%)	-	72-95

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Talin-1 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)