Product Name	Recombinant Rabbit Anti-CD38 Monoclonal Antibody
Cat No.	SLA0615M
Chinese Name	CD38单克隆抗体
Alias	ADPRC 1; ADPRC1; cADPR1; CD38_HUMAN; CD38; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1 (ADPRC 1); Cyclic ADP-ribose hydrolase 1 (cADPR hydrolase 1); T10; 3.2.2.-; 3.2.2.6; CD38 molecule; CD38 antigen (p45); ADP-ribosyl cyclase 1; NAD(+) nucleosidase
Host Species	Mouse
Isotype	IgG1
Clonality	Monoclonal
Clone No.	7H11
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1ug/Test) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human CD38 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	34 kDa
Observed MW.	45 kDa
Subcellular Location	Membrane
Gene ID	952
Swiss-Prot	P28907
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CD38 monoclonal antibody, unconjugated (SLA0615M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD38 Monoclonal Antibody, Unconjugated (SLA0615M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD38 Monoclonal Antibody, Unconjugated (SLA0615M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD38 Monoclonal Antibody, Unconjugated (SLA0615M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD38 Monoclonal Antibody, Unconjugated (SLA0615M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD38 Monoclonal Antibody, Unconjugated (SLA0615M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining
Figure 7. The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-CD38 antibody (SLA0615M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (SL60296G-FITC): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 8. 4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (CD38) monoclonal Antibody, unconjugated (SLA0615M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, SL60296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.