Product Name	Rabbit Anti-Cd36 Polyclonal Antibody
Cat No.	SLA0151
Chinese Name	CD36抗体
Alias	BDPLT10; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3; GPIIIB; IV; PAS; PAS IV; PAS-4; CD36_HUMAN; CD36; Fatty acid translocase (FAT); Glycoprotein IIIb (GPIIIB); Leukocyte differentiation antigen CD36; Platelet collagen receptor; Platelet glycoprotein IV (GPIV); Thrombospondin receptor; CD36_MOUSE; CD36_RAT; Adipocyte membrane protein; Fatty acid translocase; Fatty acid transport protein; CD36 molecule (CD36 blood group); CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); scavenger receptor class B member 3; Platelet glycoprotein 4
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Mouse
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant mouse CD36 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	52 kDa
Observed MW.	75-85 kDa
Subcellular Location	Membrane
Gene ID	12491
Swiss-Prot	Q08857
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CD36 polyclonal antibody, unconjugated (SLA0151) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated (SLA0151) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated (SLA0151) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated (SLA0151) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated (SLA0151) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 6. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated (SLA0151) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated (SLA0151) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated (SLA0151) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

• Minimum order: 1 unit
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