Product Name	Recombinant Rabbit Anti-mTOR (p-S2448) Monoclonal Antibody
Cat No.	SLA2997M
Chinese Name	磷酸化雷帕霉素靶蛋白重组兔单抗
Alias	FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; 2610315D21Rik; flat; MTOR_HUMAN; MTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin (mTOR); Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; Tyrosine-protein kinase mTOR; 2.7.11.1; MTOR_MOUSE; Rapamycin target protein 1 (RAPT1); MTOR_RAT; mechanistic target of rapamycin kinase; FK506 binding protein 12-rapamycin associated protein 1; mechanistic target of rapamycin (serine/threonine kinase); FK506 binding protein 12-rapamycin associated protein 2; rapamycin target protein; FKBP12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; rapamycin associated protein FRAP2; dJ576K7.1 (FK506 binding protein 12-rapamycin associated protein 1); mammalian target of rapamycin; mTOR (phospho-S2448); p-mTOR; phospho-mTOR; mTOR (phospho-Ser2448)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	7F5
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human mTOR around the phosphorylation site of S2448
Purification	affinity purified by Protein A
Predicted MW.	289 kDa
Observed MW.	300 kDa
Subcellular Location	Cytoplasm
Gene ID	2475
Swiss-Prot	P42345
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals.MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins.Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2).In response to nutrients, growth factors or amino acids, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis .
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. MCF-7 (H) cells were treated with or without Camptothecin (1 µM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-mTOR(S2448) monoclonal antibody, unconjugated (SLA2997M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. NIH/3T3 (M) serum-starved insulin-treated (150 nM) for 5 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-mTOR(S2448) monoclonal antibody, unconjugated (SLA2997M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (SLA2997M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (SLA2997M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (SLA2997M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (SLA2997M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (SLA2997M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with mTOR (p-S2448) Monoclonal Antibody, Unconjugated (SLA2997M) at 1:300 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with mTOR (p-S2448) Monoclonal Antibody, Unconjugated (SLA2997M) at 1:300 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.