DESCRIPTION

Leukemia inhibitory factor (LIF) is a multi-functional cytokine of the interleukin-6 (IL-6) superfamily. It utilises a receptor that consists of the LIF receptor β and gp130 and this receptor complex is also used by ciliary neurotrophic growth factor (CNTF), oncostatin M, cardiotrophin1 (CT1) and cardiotrophin-like cytokine (CLC). Functionally, LIF has been implicated in a many physiological processes including the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,138.6	39.8	3.5
2	8	294.2	5.8	2.0
3	8	143.8	5.8	4.1

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,148.9	34.8	3.0
2	16	297.1	14.2	4.8
3	16	145.9	8.0	5.5

RECOVERY

The recovery of human LIF spiked to three different levels throughout the range of the assay was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	90	82-96
Cell culture supernatant	1:32	97	80-106
	1:64	88	87-89

LINEARITY

To assess the linearity of the assay, human serum samples were spiked with high concenthumanions of human LIF and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. Cell culture supernatant samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell culture supernatant was initially diluted 1:4. )

		Human serum	Cell culture supernatant
1:2	Average% of Expected	79	100
	Range (%)	76-83	-
1:4	Average% of Expected	104	104
	Range (%)	103-105	103-105
1:8	Average% of Expected	117	105
	Range (%)	115-118	104-106
1:16	Average% of Expected	124	115
	Range (%)	120-128	109-120

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human LIF produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)