Product Name	Recombinant Rabbit Anti-MHC I Monoclonal Antibody
Cat No.	SLA2285M
Chinese Name	组织相容性抗原HLAF重组兔单抗
Alias	CDA12; HLA-5.4; HLA-CDA12; HLAF; HLAF_HUMAN; HLA-F; HLA F antigen; Leukocyte antigen F; MHC class I antigen F; major histocompatibility complex, class I, F
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	7F10
React Species	Human
Applications	WB (1:1000-2000) IHC-P (1:200-2000) IHC-F (1:200-2000) IF (1:200-2000) Flow-Cyt (1:100-200) ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human HLA F
Purification	affinity purified by Protein A
Predicted MW.	39 kDa
Observed MW.	42 kDa
Subcellular Location	Membrane
Gene ID	3134
Swiss-Prot	P30511
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Involved in the presentation of foreign antigens to the immune system.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 293T (H) cells were treated with or without PMA (50 ng/mL) for 48 h, IFN-γ (50 U/mL) for 24 h, 25 μg total protein per lane of cell lysates (see on figure) probed with HLA F monoclonal antibody, unconjugated (SLA2285M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA F Monoclonal Antibody, Unconjugated (SLA2285M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA F Monoclonal Antibody, Unconjugated (SLA2285M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA F Monoclonal Antibody, Unconjugated (SLA2285M) at 1:1500 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA F Monoclonal Antibody, Unconjugated (SLA2285M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA F Monoclonal Antibody, Unconjugated (SLA2285M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA F Monoclonal Antibody, Unconjugated (SLA2285M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA F Monoclonal Antibody, Unconjugated (SLA2285M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MHC I | HLA-F Monoclonal Antibody, Unconjugated (SLA2285M) at 1:1500 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining	Figure 10. The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-HLA F antibody (SLA2285M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 11. 4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (HLA F) monoclonal Antibody, unconjugated (SLA2285M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.