Product Name	Recombinant Rabbit Anti-RACK1 Monoclonal Antibody
Cat No.	SLA1946M
Chinese Name	受体激活蛋白激酶C1重组兔单抗
Alias	C12-3; GNB2L1; c12.3; Gnb2-rs1; H12.3; HLC-7; PIG21; GB-like; p205; RACK1_CHICK; RACK1; Guanine nucleotide-binding protein subunit beta-like protein 12.3; Receptor for activated C kinase; Receptor of activated protein C kinase 1; Receptor of activated protein kinase C 1 (RACK1); RACK1_BOVIN; Guanine nucleotide-binding protein subunit beta-2-like 1; Receptor of activated protein kinase C 1; GBLP_MACFA; RACK1_HUMAN; Cell proliferation-inducing gene 21 protein; Human lung cancer oncogene 7 protein (HLC-7); RACK1_MOUSE; 12-3; RACK1_PIG; RACK1_RAT; receptor for activated C kinase 1; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	9B3
React Species	Human,Mouse,Rat
Applications	IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human RACK1
Purification	affinity purified by Protein A
Predicted MW.	35 kDa
Observed MW.	35 kDa
Subcellular Location	Nucleus
Gene ID	10399
Swiss-Prot	P63244
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RACK1 Monoclonal Antibody, Unconjugated (SLA1946M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RACK1 Monoclonal Antibody, Unconjugated (SLA1946M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RACK1 Monoclonal Antibody, Unconjugated (SLA1946M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RACK1 Monoclonal Antibody, Unconjugated (SLA1946M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RACK1 Monoclonal Antibody, Unconjugated (SLA1946M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RACK1 Monoclonal Antibody, Unconjugated (SLA1946M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RACK1 Monoclonal Antibody, Unconjugated (SLA1946M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RACK1 Monoclonal Antibody, Unconjugated (SLA1946M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RACK1 Monoclonal Antibody, Unconjugated (SLA1946M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RACK1 | GNB2L1 Monoclonal Antibody, Unconjugated (SLA1946M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RACK1 | GNB2L1 Monoclonal Antibody, Unconjugated (SLA1946M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.