Product Name	Recombinant Rabbit Anti-DNMT1 Monoclonal Antibody
Cat No.	SLA1480M
Chinese Name	DNA甲基转移酶1重组兔单抗
Alias	DNMT1_BOVIN; DNMT1; 2.1.1.37; DNMT1_HUMAN; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI (DNA MTase HsaI | M.HsaI); MCMT; AIM; CXXC9; DNMT; DNMT1_MOUSE; Met-1; DNA methyltransferase MmuI (DNA MTase MmuI | M.MmuI); Met1; Uim; DNMT1_RAT; DNA MTase RnoIP (M.RnoIP); DNA methyltransferase I; DNA methyltransferase 1; DNA (cytosine-5-)-methyltransferase 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	1B3
React Species	Human,Mouse,Rat
Applications	WB (1:500-1000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human DNMT1
Purification	affinity purified by Protein A
Predicted MW.	183 kDa
Observed MW.	220 kDa
Subcellular Location	Nucleus
Gene ID	1786
Swiss-Prot	P26358
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Dnmt1 monoclonal antibody, unconjugated (SLA1480M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(SLA1480M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(SLA1480M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(SLA1480M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(SLA1480M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(SLA1480M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(SLA1480M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(SLA1480M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Mouse testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(SLA1480M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(SLA1480M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(SLA1480M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(SLA1480M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.