Summary:

Purpose

Our Quick Step Canine anti-hepatitis B virus core antibody(HBcAb) ELISA Kit is to for the qualitative determination of HBcAb in Canine serum, plasma, culture media or any biological fluid.

Principle

The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an antigen specific toHBcAb, make it to solid-phase antigen.Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for HBcAb is added to each Microplate well and incubated,so the antigen-antibody-Enzyme labeled antigen complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain HBcAb and HRP conjugated HBc antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of HBcAb is determined by comparing with the CUT OFF value.

Determine the result

Test effectiveness: the average value of positive control ≥1.00; The average value

of negative control ≤0.10.

The critical value (CUT OFF) calculation: critical value = the average value of

negative control + 0.15

Negative judgement: if the OD value<CUT OFF, the sample is Canine HBcAb negative.

Positive judgement: if the OD value ≥CUT OFF, the sample is Canine HBcAb positive.