Summary:

Purpose

Our Quick Step Horse tumor necrosis factor α(TNF-α) ELISA Kit is to assay TNF-α levels in Horse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TNF-α. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for TNF-α is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TNF-α and HRP conjugated TNF-α antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TNF-α. You can calculate the concentration of TNF-α in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0039Ho is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.048	0.0505
Blank Well	0pg/ml	0.053	0.0505
S1	180pg/ml	1.941	1.9370
S1	180pg/ml	1.933	1.9370
S2	120pg/ml	1.317	1.3255
S2	120pg/ml	1.334	1.3255
S3	60pg/ml	0.709	0.7200
S3	60pg/ml	0.731	0.7200
S4	30pg/ml	0.373	0.3645
S4	30pg/ml	0.356	0.3645
S5	15pg/ml	0.187	0.1900
S5	15pg/ml	0.193	0.1900