Summary:

Purpose

Our Quick Step Horse Prostaglandin F2 Alpha (PGF2a) ELISA Kit is to assay PGF2a levels in Horse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PGF2a. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for PGF2a is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PGF2a and HRP conjugated PGF2a antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PGF2a. You can calculate the concentration of PGF2a in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0037Ho is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.048	0.0505
Blank Well	0pg/ml	0.053	0.0505
S1	180pg/ml	1.941	1.9370
S1	180pg/ml	1.933	1.9370
S2	120pg/ml	1.317	1.3255
S2	120pg/ml	1.334	1.3255
S3	60pg/ml	0.709	0.7200
S3	60pg/ml	0.731	0.7200
S4	30pg/ml	0.373	0.3645
S4	30pg/ml	0.356	0.3645
S5	15pg/ml	0.187	0.1900
S5	15pg/ml	0.193	0.1900