DESCRIPTION

CCL5, also known as RANTES, belongs to the CC chemokine family. It is strongly expressed in the human and murine liver upon injury. CCL5 has been found to be produced by numerous cell types, including platelets, macrophages, endothelial, and stellate cells. CCL5 recruits T cells, dendritic cells, eosinophils, NK cells, mast cells, and basophils to sites of inflammation by interacting with 3 specific G protein-coupled receptors: CCR1, CCR3, and CCR5. While CCL5 may promote efficient anti-tumour immune responses, it has also been associated with cancer progression and metastasis.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	7.44	0.15	2.02
2	8	1.26	0.02	1.59
3	8	0.67	0.02	2.99

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	7.39	0.21	2.84
2	16	1.27	0.02	1.57
3	16	0.68	0.02	2.94

RECOVERY

The recovery of mouse/rat CCL5/RANTES spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:2	112	107-123
Rat serum	1:16	120	115-125
	1:32	114	107-120

LINEARITY

To assess the linearity of the assay, mouse serum samples were spiked with high concentrations of mouse CCL5/RANTES and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. Rat serum samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The rat serum was initially diluted 1:2.)

		Mouse serum(Sample Diluent PT 4)	Rat serum(Sample Diluent PT 1)
1:2	Average% of Expected	84	100
	Range (%)	72-95	-
1:4	Average% of Expected	95	106
	Range (%)	83-107	100-112
1:8	Average% of Expected	108	105
	Range (%)	86-129	95-116
1:16	Average% of Expected	107	108
	Range (%)	86-129	102-114

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Mouse/Rat CCL5/RANTES One-Step ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)