DESCRIPTION

GPR56 (also known as ADGRG1) is an adhesion G protein‑coupled receptor (aGPCR) that contains an N‑terminal adhesion domain, a GAIN domain, seven transmembrane helical regions, and a C‑terminal intracellular tail. This protein regulates cell adhesion, migration, and differentiation through the Gα12/13 and RhoA signaling pathways. GPR56 is a critical regulator of cortical development; its mutations cause bilateral frontoparietal polymicrogyria (BFPP), characterized by abnormal cortical folding and intellectual disability. In cancer, GPR56 exerts a metastasis‑suppressive role in melanoma and glioblastoma, but promotes tumor progression in acute myeloid leukemia.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,796.8	52.2	2.9
2	8	452.4	13.4	3.0
3	8	207.4	10.7	5.2

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,781.5	49.4	2.8
2	16	440.9	18.9	4.3
3	16	206.2	14.1	6.8

RECOVERY

The recovery of human GPR56 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:4	85	76-93
	1:8	97	89-101
Cell lysate	1:2	97	90-102
	1:4	99	98-101

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum(Sample Diluent PT 4B1)	Cell lysate(Sample Diluent PT 5)
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	116	104
	Range (%)	115-116	103-104
1:8	Average% of Expected	109	103
	Range (%)	100-118	101-105
1:16	Average% of Expected	108	107
	Range (%)	105-111	105-110

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human GPR56 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)