DESCRIPTION

Angiotensinogen(AGT), a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	5.42	0.13	2.40
2	8	1.27	0.04	3.15
3	8	0.66	0.02	3.03

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	5.46	0.11	2.01
2	16	1.27	0.03	2.36
3	16	0.66	0.02	3.03

RECOVERY

The recovery of human Angiotensinogen/AGT spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:6,400	116	112-120
	1:12,800	109	107-112
Urine	1:8	119	107-127

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum was initially diluted 1:800.)

		Human serum	Urine
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	90	101
	Range (%)	88-91	94-107
1:8	Average% of Expected	83	99
	Range (%)	79-88	88-110
1:16	Average% of Expected	92	97
	Range (%)	88-97	84-110

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human Angiotensinogen/AGT One-Step ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)