Product Name	Rabbit Anti-NKX6-1 Polyclonal Antibody
Cat No.	SLA0013
Chinese Name	转录因子相关蛋白NKX6.1抗体
Alias	NKX61_HUMAN; NKX6-1; Homeobox protein NK-6 homolog A; NKX6A; NKX61_MOUSE; Nkx6.1; NK6 homeobox 1; NK homeobox (Drosophila), family 6, A; NK6 transcription factor related, locus 1 (Drosophila)
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Mouse
Applications	WB (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from Human NKX6.1
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	38 kDa
Observed MW.	52 kDa
Subcellular Location	Nucleus
Gene ID	4825
Swiss-Prot	P78426
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Members of the Nkx family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart and pancreas. During neural development, sonic hedgehog (Shh) is known to control cell fate and mitogenesis, which is correlated with Shh dose-dependent expression of several genes, including Nkx-6.1. Specifically, Nkx-6.1 is responsible for cellular differentiation in the ventral neural tube and spinal meninges in response to Shh. In the pancreas, Nkx-6.1 is exclusively expressed in the islets of Langerhans in differentiating and mature b cells, which produce insulin. The presence of Pdx-1 is required for the expression of Nkx-6.1 as well as other pancreatic b cell specific genes, including insulin, Glut2 and IAPP. Subsequently, Nkx-6.1 binds to the DNA consensus sequence, TTAATTAC, to direct the repression of specific genes in b cells.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Mouse Pancreas tissue lysates Primary: Anti-NKX6.1 (SLA0013) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 52 kDa

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.