DESCRIPTION

CD44 is a transmembrane cell surface glycoprotein. It is primarily involved in regulating cell adhesion, migration, angiogenesis, proliferation, and inflammation. It may take part in the process of tumorigenesis as CD44 and its variants have a potential role in the development of malignant tumors. CD44 has gained significant attention because of its usefulness as a stem cell marker and has surfaced as a budding therapeutic target, imposing a greater understanding of CD44 in various cancers. Additionally, Serum CD44 concentration correlated with tumor burden and metastasis of tumors.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	7.21	0.17	2.36
2	8	2.23	0.05	2.24
3	8	1.33	0.02	1.50

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	7.24	0.16	2.21
2	16	2.24	0.05	2.23
3	16	1.35	0.04	2.96

RECOVERY

The recovery of mouse CD44 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:32	99	92-113
	1:64	93	93-95
Tissue lysate	1:16	94	88-98
	1:32	85	77-93

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:4. The tissue lysate was initially diluted 1:2.)

		Cell lysate(Sample Diluent PT 5-ef)	Tissue lysate(Sample Diluent PT 3)
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	100	84
	Range (%)	92-107	82-86
1:8	Average% of Expected	108	87
	Range (%)	93-123	80-93
1:16	Average% of Expected	109	96
	Range (%)	91-128	83-110

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CD44 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)