DESCRIPTION

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia. TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles. It associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	292.2	5.9	2.0
2	8	67.3	2.2	3.3
3	8	38.4	0.8	2.1

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	288.4	7.2	2.5
2	16	66.2	2.0	3.0
3	16	37.8	1.1	2.9

RECOVERY

The recovery of human TREM2 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:160	96	95-97
	1:320	111	105-118
Cell culture supernatant	1:80	96	83-107
Cell lysate	1:40	91	87-95
Human cerebrospinal fluid (CSF)	1:80	106	105-109

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma was initially diluted 1:20. The cell culture supernatant was initially diluted 1:5. The cell lysate was initially diluted 1:2.5. The human cerebrospinal fluid (CSF) was initially diluted 1:5.)

		Human plasma	Cell culturesupernatant	Cell lysate	Humancerebrospinal fluid(CSF)
1:2	Average% of Expected	100	100	100	100
	Range (%)	-	-	-	-
1:4	Average% of Expected	99	96	83	106
	Range (%)	98-100	92-100	75-91	103-108
1:8	Average% of Expected	86	89	94	101
	Range (%)	75-97	87-91	82-107	100-103
1:16	Average% of Expected	87	92	101	99
	Range (%)	81-93	87-98	90-113	97-102

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human TREM2 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)