DESCRIPTION

Triggering receptor expressed on myeloid cells-1 (TREM-1, also known as CD354) is a member of the immunoglobulin superfamily. It plays a crucial role in the innate immune response to bacterial and fungal infections by triggering the release of pro-inflammatory chemokines and cytokines and increasing the surface expression of cell activation markers. TREM-1 also exists as a soluble receptor (sTREM-1) that arises from proteolytic cleavage of the membrane-bound receptor by matrix metalloproteases. The serum levels of sTREM-1 may correlate with disease severity and treatment outcomes in pulmonary tuberculosis patients.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	497.1	13.8	2.8
2	8	124.9	2.8	2.2
3	8	67.6	2.4	3.6

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	488.5	15.9	3.3
2	16	123.7	2.8	2.3
3	16	66.3	2.8	4.2

RECOVERY

The recovery of human TREM-1/CD354 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:8	107	103-112
Cell culture supernatant	1:8	106	100-111
Saliva	1:4	114	99-123
Cell lysate	1:16	98	95-100

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human plasma(Sample Diluent PT1)	Cell culturesupernatant(Sample Diluent PT4B1)	Saliva(Sample Diluent PT1)	Cell lysate(Sample Diluent PT5)
1:2	Average% of Expected	100	100	100	100
	Range (%)	-	-	-	-
1:4	Average% of Expected	124	93	97	84
	Range (%)	123-124	92-93	85-109	82-86
1:8	Average% of Expected	114	89	91	95
	Range (%)	112-117	87-92	81-102	91-99
1:16	Average% of Expected	102	92	94	85
	Range (%)	98-106	81-104	82-105	82-87

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human TREM-1/CD354 One-Step ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)