DESCRIPTION

PAK4 (p21-activated kinase 4) is a serine/threonine kinase that plays a crucial role in regulating various cellular processes, including cell proliferation, survival, migration, and cytoskeletal dynamics. PAK4 is involved in several signaling pathways that are important for cellular homeostasis and development. Dysregulation of PAK4 activity has been implicated in various diseases, particularly cancer, where it contributes to tumorigenesis by promoting cell survival and migration. Additionally, PAK4 is thought to participate in the regulation of neurogenesis and synaptic plasticity. Given its pivotal role in cell signaling and disease, PAK4 has become a potential target for therapeutic interventions in cancer and other disorders. Inhibition of PAK4 has been shown to impair tumor growth in preclinical models, making it a promising candidate for drug development.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	21.64	0.49	2.26
2	8	5.67	0.14	2.47
3	8	3.23	0.08	2.48

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	21.87	0.65	2.97
2	16	5.68	0.11	1.94
3	16	3.28	0.10	3.05

RECOVERY

The recovery of human PAK4 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:2	109	103-113
	1:4	115	106-120

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	99
	Range (%)	94-104
1:8	Average% of Expected	107
	Range (%)	107-108
1:16	Average% of Expected	108
	Range (%)	107-109

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human PAK4 One-Step ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)