DESCRIPTION

Neuropilin-1 (NRP1) is a transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 has been demonstrated to play a role in the stability and function of Tregs. It interacts with the ligand Semaphorin-4a (Sema4a) expressed on Tregs to enhance the function and survival of Tregs in tumors, restricting the anti-tumor immune response. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. In summary, NRP1 is a multifunctional receptor protein that is involved in nerve, blood vessel, and tumor development, with implications in immune regulation, tumor growth, and angiogenesis.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,973.5	118.6	4.0
2	8	744.2	15.0	2.0
3	8	361.0	9.1	2.5

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	3,005.8	200.6	6.7
2	16	742.6	21.7	2.9
3	16	360.3	10.2	2.8

RECOVERY

The recovery of human Neuropilin 1/CD304 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:640	106	101-109
Cell culture supernatant	1:8	113	110-115
	1:16	109	100-114
Urine	1:2	119	116-121
Human milk	1:80	97	82-106
	1:160	116	113-122

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma was initially diluted 1:80. The human milk was initially diluted 1:20.)

		Human plasma	Cell culturesupernatant	Urine	Human milk
1:2	Average% of Expected	100	100	100	100
	Range (%)	-	-	-	-
1:4	Average% of Expected	97	88	112	105
	Range (%)	94-100	87-89	110-115	102-108
1:8	Average% of Expected	98	90	108	99
	Range (%)	96-101	89-91	100-116	98-99
1:16	Average% of Expected	90	85	95	98
	Range (%)	81-100	85-86	89-101	92-104

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human Neuropilin 1/CD304 One-Step ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)