DESCRIPTION

CD5 antigen (CD5, also known as Ly-1, Ly-A, Ly-12, and Lyt-1) is a pan-T cell marker and a negative regulator of TCR and BCR signaling, which increases expression during T-cell development in the thymus. It is associated with the B cell receptor (BCR) complex and is a substrate for BCR-induced tyrosine kinase activity. In addition to membrane-bound CD5, the circulating soluble form of CD5 (sCD5) has been shown in sera resulting from proteolytic cleavage following lymphocyte activation. The expression level of sCD5 is associated with the development of autoimmune and inflammatory disorders.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,044.1	36.2	3.5
2	8	259.5	12.5	4.8
3	8	139.6	7.9	5.7

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,093.7	85.4	7.8
2	16	257.4	10.8	4.2
3	16	141.0	7.4	5.2

RECOVERY

The recovery of human CD5 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	104	94-110
Cell lysate	1:800	100	96-106
	1:1,600	106	101-109

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:100.)

		Human serum(Sample Diluent PT 5)	Cell lysate(Sample Diluent PT 4B1)
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	93	104
	Range (%)	91-96	101-108
1:8	Average% of Expected	88	104
	Range (%)	82-93	100-107
1:16	Average% of Expected	77	102
	Range (%)	70-83	101-102

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human CD5 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)