Aliases:	SUPT5H; SPT5 Homolog, DSIF Elongation Factor Subunit; SPT5H; SPT5; DRB Sensitivity-Inducing Factor 160 KDa Subunit; DRB Sensitivity-Inducing Factor Large Subunit; Transcription Elongation Factor SPT5; Tat-Cotransactivator 1 Protein; DSIF Large Subunit; DSIF P160; FLJ34157; HSPT5; Suppressor Of Ty 5 Homolog (S. Cerevisiae); Suppressor Of Ty (S.Cerevisiae) 5 Homolog; Suppressor Of Ty 5 Homolog; Tat-CT1 Protein; Tat-CT1
Background:	UniProt Entry: O00267;NCBI Gene Entry: 6829
Application Information	Molecular Weight: Predicted, 121 kDa, observed, 155 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4220 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SUPT5H
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of SUPT5H expression in HT-1080 cells using SUPT5H antibody 1:2,000. Green, isotype control; red, SUPT5H.

Immunocytochemical staining of HT-1080 cells with SUPT5H antibody 1:1,000. Nuclei were stained blue with DAPI; SUPT5H was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-SUPT5H antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-SUPT5H antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.