DESCRIPTION

CD117, also known as c-Kit, is a type III receptor tyrosine kinase that plays a crucial role in cell signal transduction. It is activated by binding to its ligand, stem cell factor (SCF), leading to a phosphorylation cascade that activates various transcription factors in different cell types. This activation regulates apoptosis, cell differentiation, proliferation, chemotaxis, and cell adhesion. C-Kit is essential for the development and function of several cell types, including mast cells, hematopoietic stem cells, germ cells, melanocytes, and interstitial cells of Cajal in the gastrointestinal tract. It is also involved in processes such as hematopoiesis, gametogenesis, melanogenesis, and gastrointestinal motility.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	10.37	0.21	2.03
2	8	2.66	0.08	3.01
3	8	1.27	0.04	3.15

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	10.43	0.26	2.49
2	16	2.62	0.08	3.05
3	16	1.29	0.06	4.65

RECOVERY

The recovery of human CD117/c-Kit spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:400	101	100-102
	1:800	96	93-101
Cell culture supernatant	1:2	96	96-97
	1:4	99	98-100

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma was initially diluted 1:50. )

		Human plasma	Cell culture supernatant
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	104	105
	Range (%)	103-104	103-107
1:8	Average% of Expected	104	114
	Range (%)	103-105	106-121
1:16	Average% of Expected	105	-
	Range (%)	105-106	-

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human CD117/c-Kit One-Step ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)