DESCRIPTION

CD79 is composed of CD79a and CD79b components expressed almost exclusively on B cells and B-cell neoplasms. The transmembrane CD79a and CD79b proteins couple at the extracellular end with any one of the five different types of transmembrane Ig molecules (IgM, IgD, IgG, IgE, or IgA), which are disulfide-linked proteins composed of two Ig heavy chains and two Ig light chains. The intracytoplasmic ends of the CD79a/CD79b proteins can couple with intracellular signaling components as well. Furthermore, Activated B lymphocytes have increased amounts of the truncated CD79b. Loss of CD79b expression is a characteristic feature of CLL/SLL and may be of diagnostic use.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,759.4	78.0	2.8
2	8	824.6	19.2	2.3
3	8	232.2	21.3	9.2

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,681.4	131.2	4.9
2	16	769.8	61.5	8.0
3	16	230.1	20.8	9.0

RECOVERY

The recovery of mouse CD79B spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Tissue lysate	1:640	95	90-98
Tissue homogenate	1:40	94	89-100
	1:80	99	96-105

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The tissue lysate was initially diluted 1:80. The tissue homogenate was initially diluted 1:5.)

		Tissue lysate	Tissue homogenate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	99	98
	Range (%)	97-100	95-101
1:8	Average% of Expected	107	99
	Range (%)	103-110	92-106
1:16	Average% of Expected	113	101
	Range (%)	103-122	84-118

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CD79B produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)