DESCRIPTION

CD74 is a non-polymorphic type II transmembrane glycoprotein. It is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. Additionally, CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,162.3	65.1	3.0
2	8	622.9	13.3	2.1
3	8	364.2	8.9	2.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,205.5	112.9	5.1
2	16	629.1	21.3	3.4
3	16	364.2	9.8	2.7

RECOVERY

The recovery of mouse CD74 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:32	100	95-103
	1:64	102	97-107
Tissue lysate	1:64	108	105-113
	1:128	106	98-115

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The mouse serum was initially diluted 1:4. The tissue lysate was initially diluted 1:256.)

		Mouse serum(Sample Diluent PT 3)	Tissue lysate(Sample Diluent PT 4B1)
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	94	102
	Range (%)	93-95	101-103
1:8	Average% of Expected	86	109
	Range (%)	85-86	103-115
1:16	Average% of Expected	79	106
	Range (%)	78-80	104-107

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CD74 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)