DESCRIPTION

CD31, also known as platelet endothelial cell adhesion molecule1 (PECAM-1), is a 130 kDa platelet-endothelial cell (EC) adhesion molecule initially identified from ECs and platelets and later from blood leucocytes. CD31 was initially classified as a cell adhesion molecule, later studies suggested that CD31 triggers downstream inhibitory signaling upon trans-homophilic CD31 engagement during cell-cell interaction. CD31 signaling participates in regulating leucocyte detachment, T-cell activation, platelet activation, and angiogenesis, all of which are critical to the pathogenesis of atherosclerosis and abdominal aortic aneurysms (AAAs).

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	669.0	30.3	4.5
2	8	181.7	6.5	3.6
3	8	49.3	3.5	7.0

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	676.3	25.0	3.7
2	16	182.2	10.8	5.9
3	16	51.4	3.8	7.4

RECOVERY

The recovery of mouse CD31 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:64	95	84-118
Cell lysate	1:8	92	87-98
Tissue lysate	1:128	99	94-105

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The mouse serum was initially diluted 1:8. The tissue lysate was initially diluted 1:128.)

		Mouse serum(Sample Diluent PT 5)	Cell lysate(Sample Diluent PT 3-ef)	Tissue lysate(Sample Diluent PT 4B1)
1:2	Average% of Expected	100	100	100
	Range (%)	-	-	-
1:4	Average% of Expected	97	84	101
	Range (%)	86-109	77-91	96-107
1:8	Average% of Expected	102	90	98
	Range (%)	96-109	90-91	96-100
1:16	Average% of Expected	109	88	97
	Range (%)	105-113	80-97	96-98

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CD31 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)