DESCRIPTION

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1 alpha, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with biotin) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with biotin also binds to the analyte. Streptavidin-HRP binds to the biotin. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	86.8	3.8	4.4
2	20	25.8	0.9	3.5
3	20	5.3	0.1	2.5

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	83.8	3.9	4.7
2	24	25.7	0.8	3.0
3	24	5.1	0.3	5.4

RECOVERY

The recovery of mouse CCL3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:2	103	75-123
	1:4	111	94-126
Cell culture supernatant	1:2	103	89-113
	1:4	104	87-123

LINEARITY

To assess the linearity of the assay, mouse serum and cell culture supernatant samples were spiked with high concentrations of mouse CCL3 and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Mouse serum(Sample Diluent PT 1-ac)	Cell culture supernatant(Sample Diluent PT 1-ef)
1:2	Average% of Expected	89	84
	Range (%)	72-100	72-97
1:4	Average% of Expected	105	85
	Range (%)	100-115	72-97
1:8	Average% of Expected	111	86
	Range (%)	96-123	74-98
1:16	Average% of Expected	110	90
	Range (%)	101-123	81-99

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CCL3 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)