Aliases:	USP14; Ubiquitin Specific Peptidase 14; TGT; Ubp6; Ubiquitin Specific Peptidase 14 (TRNA-Guanine Transglycosylase); Ubiquitin Specific Protease 14 (TRNA-Guanine Transglycosylase); Ubiquitin Carboxyl-Terminal Hydrolase 14; Deubiquitinating Enzyme 14; Ubiquitin Thioesterase 14; TRNA-Guanine Transglycosylase, 60-KD Subunit; Ubiquitin-Specific Processing Protease 14; Ubiquitin-Specific-Processing Protease 14; Ubiquitin Thiolesterase 14; EC 3.4.19.12; EC 3.1.2.15
Background:	UniProt Entry: P54578;NCBI Gene Entry: 9097
Application Information	Molecular Weight: Predicted, 56 kDa, observed, 56 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2190 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human USP14
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Ubiquitin specific peptidase 14 expression in HepG2 cells using anti-Ubiquitin specific peptidase 14 antibody 1:2,000. Green, isotype control; red, Ubiquitin specific peptidase 14.

Immunocytochemical staining of HepG2 cells with anti-Ubiquitin specific peptidase 14 antibody 1:1,000. Nuclei were stained blue with DAPI; Ubiquitin specific peptidase 14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-ubiquitin specific peptidase 14 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Ubiquitin specific peptidase 14 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .