DESCRIPTION

ASC/TMS1, also known as CARD5. The calculated molecular weight of ASC/TMS1 is 21 kDa. It is located in cytosol, extracellular region and nucleus. ASC/TMS1 is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,288.2	51.61	4.0
2	8	324.5	10.50	3.2
3	8	106.0	4.66	4.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,277.8	53.1	4.2
2	16	318.7	10.9	3.4
3	16	107.8	5.3	4.9

RECOVERY

The recovery of mouse ASC/TMS1 spiked to three different levels throughout the range of the assay in tissue lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Tissue lysate	1:640	82	74-86
	1:1,280	84	77-91

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The tissue lysate was initially diluted 1:80.)

		Tissue lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	106
	Range (%)	101-109
1:8	Average% of Expected	111
	Range (%)	106-117
1:16	Average% of Expected	114
	Range (%)	109-120

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Mouse ASC/TMS1 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)