DESCRIPTION

Apolipoprotein E (APOE) is a core component of plasma lipoproteins and is involved in a variety of biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immune regulation, and neuronal growth and repair. The ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). Mouse APOE, like ApoE4, contains equivalents of Arg-112 and Glu-255 but lacks the critical Arg-61 equivalent. APOE has heparin-binding activity and binds to heparan sulfate proteoglycans on the cell surface, a property that facilitates cellular capture and receptormediated uptake of APOE-containing lipoproteins.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	20	6.14	0.30	4.90
2	20	1.12	0.03	2.78
3	20	0.38	0.02	4.52

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	24	6.66	0.59	8.93
2	24	1.14	0.03	2.47
3	24	0.41	0.02	3.73

RECOVERY

The recovery of mouse APOE spiked to three different levels throughout the range of the assay in mouse serum was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:12,800	100	94-120

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The mouse serum samples were initially diluted 1:8,000.)

		Mouse serum
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	91
	Range (%)	89-95
1:8	Average% of Expected	87
	Range (%)	77-94
1:16	Average% of Expected	102
	Range (%)	98-108

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse APOE produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)