DESCRIPTION

Y-box binding protein 1 (YBX1), also known as YB-1, is a multifunctional member of the cold shock domain protein family and serves as a significant transcription factor. It regulates transcription by binding to Y-box sequences found in the enhancer and promoter regions of various genes, thereby modulating the expression of numerous genes associated with cellular proliferation, senescence, survival, drug resistance, and chromosomal instability. This regulation implicates YBX1 in a broad spectrum of biological processes, such as apoptosis, cell proliferation, differentiation, and stress response. Additionally, YBX1 is a key member of the RNA-binding protein (RBP) family, playing an important role in regulating RNA modifications and metabolism.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,194.1	48.8	4.1
2	8	314.1	15.8	5.0
3	8	157.5	5.5	3.5

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,150.2	66.4	5.8
2	16	311.3	20.4	6.6
3	16	155.7	7.8	5.0

RECOVERY

The recovery of human YBX1 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:64	77	75-80

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:4.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	91
	Range (%)	83-98
1:8	Average% of Expected	95
	Range (%)	70-121
1:16	Average% of Expected	106
	Range (%)	87-124

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human YBX1 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)