DESCRIPTION

VEGFR2, also named as KDR, FLK1 and CD309, is one of the two tyrosine kinase receptors involved in angiogenesis. VEGFR2 is mainly distributed in vascular endothelial cells, lymphatic endothelial cells and embryonic precursor cells, and can bind to VEGF-A, VEGF-C and VEGF-D. When activated by its ligand VEGF, VEGFR2 promotes neighbouring vessel formation to facilitate the delivery of growth factors, nutrients and oxygen for cancer proliferation, migration, metastasis and survival. VEGF and VEGFR2 mediated angiogenesis contributes to the aggressive natures and leads to high mortality rate in gastric cancer.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	2,460.0	137.7	5.6
2	20	613.1	39.9	6.5
3	20	172.1	11.0	6.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	2,710.6	254.5	9.4
2	24	624.5	40.7	6.5
3	24	184.2	13.3	7.2

RECOVERY

The recovery of human VEGFR2/KDR spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:64	97	93-105
Cell culture supernatant	1:2	99	95-105
	1:4	87	77-96
Cell lysate	1:128	92	90-93
	1:256	99	88-112

LINEARITY

To assess the linearity of the assay, cell culture supernatant samples were spiked with high concentrations of human VEGFR2/KDR and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. Human serum and cell lysate samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum was initially diluted 1:4. The cell lysate was initially diluted 1:16.)

		Human serum(Sample Diluent PT 1)	Cell culture supernatant(Sample Diluent PT 4B1)	Cell lysate(Sample Diluent PT 4B1)
1:2	Average% of Expected	100	92	100
	Range (%)	-	88-94	-
1:4	Average% of Expected	97	97	98
	Range (%)	96-98	94-100	93-107
1:8	Average% of Expected	99	91	102
	Range (%)	97-100	84-95	94-111
1:16	Average% of Expected	106	85	95
	Range (%)	104-107	79-93	85-115

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human VEGFR2/KDR Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)