DESCRIPTION

Vascular endothelial growth factor-D (VEGF-D) is a secreted glycoprotein that can activate VEGF receptors on the endothelium, is a mitogen for endothelial cells and promotes the growth and remodeling of blood vessels and lymphatic vessels. VEGF-D undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR3 receptors. VEGF-D has been established to be over-expressed in both tumor tissues and patients' serum samples in several types of human cancer.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with biotin) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with biotin also binds to the analyte. Streptavidin-HRP binds to the biotin. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	1,028.8	63.9	6.2
2	20	240.2	11.3	4.7
3	20	56.5	3.5	6.2

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	1,060.9	45.8	4.3
2	24	263.7	13.5	5.1
3	24	61.6	3.2	5.1

RECOVERY

The recovery of human VEGF-D spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:4	98	76-113
	1:8	102	91-113
Cell culture supernatant	1:2	111	105-119
	1:4	112	87-128

LINEARITY

To assess the linearity of the assay, human plasma samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. Cell culture supernatant was spiked with high concentrations of human VEGF-D in various matrices and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human plasma	Cell culture supernatant
1:2	Average% of Expected	100	99
	Range (%)	-	95-102
1:4	Average% of Expected	118	102
	Range (%)	107-128	97-108
1:8	Average% of Expected	101	110
	Range (%)	93-112	107-116
1:16	Average% of Expected	99	107
	Range (%)	87-110	103-110

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human VEGF-D produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)