DESCRIPTION

Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), also identified as DcR3 or M68, is a secreted member of the Tumor Necrosis Factor (TNF) receptor superfamily, neutralizes three different TNF ligands: TNFS14/LIGHT, TNFSF15/TL1A and TNFSF6/FasL. TNFRSF6B binds to FasL and inhibits FasL-induced apoptosis. It can also bind to LIGHT and suppresse LIGHTmediated apoptosis. Interaction of TL1A with DR3 promotes T cell expansion during an immune response. Expression of DcR3 is significantly elevated in patients with a range of cancers and autoimmune diseases.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with biotin) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with biotin also binds to the analyte. Streptavidin-HRP binds to the biotin. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	1,145.1	27.5	2.4
2	20	264.4	5.0	1.9
3	20	62.8	2.3	3.7

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	1,200.1	24.8	2.1
2	24	262.6	6.1	2.3
3	24	55.6	2.3	4.2

RECOVERY

The recovery of human TNFRSF6B/DcR3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:60	85	75-96
	1:120	82	75-90

LINEARITY

To assess the linearity of the assay, cell lysate was diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.(The cell lysate was initially diluted 1:16)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	111
	Range (%)	108-113
1:8	Average% of Expected	108
	Range (%)	99-121

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human TNFRSF6B/DcR3 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)