DESCRIPTION

TGF-beta is a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. TGF-beta is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells. TGF-beta acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. TGF-beta plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. TGF-beta appears to promote late stage progression and metastasis in some cancers.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with biotin) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with biotin also binds to the analyte. Streptavidin-HRP binds to the biotin. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	632.6	28.2	4.5
2	20	81.2	3.9	4.8
3	20	18.5	0.9	4.9

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	659.6	62.0	9.4
2	24	83.3	4.5	5.5
3	24	15.5	0.3	2.0

RECOVERY

The recovery of human TGF-beta1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:50	94	81-110
	1:100	106	103-111
Cell culture supernatant	1:10	101	93-111
	1:20	109	104-115
Urine	1:5	94	86-101
	1:10	92	87-96

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The serum and plasma samples were initially diluted 1:50. The cell culture supernatant samples was initially diluted 1:1)

		Human plasma	Cell culture supernatant	Urine
1:2	Average% of Expected	85	94	100
	Range (%)	81-91	84-107	95-109
1:4	Average% of Expected	95	96	98
	Range (%)	91-101	89-101	87-110
1:8	Average% of Expected	99	97	98
	Range (%)	97-102	90-103	89-109
1:16	Average% of Expected	103	103	98
	Range (%)	96-110	96-111	91-107

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human TGF-beta1 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)