DESCRIPTION

Sialic acid-binding Ig-like lectin 7 (Siglec-7, also known as CD328) is an inhibitory receptor expressed on natural killer (NK) cells, monocytes, macrophages, dendritic cells, and mast cells. It plays a crucial role in regulating immune responses by binding to sialic acid-containing glycans on the surface of target cells, including cancer cells and pathogens. Moreover, Siglec-7 is emerging as a very important regulator of the immune response, which shows that increased expression of sialylated ligands on tumour cells inhibits NK cell activation through engagement of Siglec-7.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,189.2	40.0	3.4
2	8	297.8	7.0	2.4
3	8	161.3	3.4	2.1

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,246.5	66.3	5.3
2	16	309.4	13.3	4.3
3	16	169.7	10.2	6.0

RECOVERY

The recovery of human Siglec-7/CD328 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:4	98	91-107
	1:8	90	86-95
Lysate	1:16	105	102-110
	1:32	100	95-106

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The lysate was initially diluted 1:2.)

		Human serum	Lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	104	110
	Range (%)	102-105	104-116
1:8	Average% of Expected	103	91
	Range (%)	99-108	87-95
1:16	Average% of Expected	110	82
	Range (%)	106-114	81-83

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human Siglec-7/CD328 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)