DESCRIPTION

PARP14 (poly (ADP-ribose) polymerase family, member 14) is a PARP family member that has roles in DNA repair, B cell regulation, and focal adhesion. The largest of all the human PARPs is PARP14. PARP14 has been reported to regulate several different pathways involved in immunity, inflammation, and genome stability.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	24.44	0.86	3.51
2	8	6.35	0.20	3.12
3	8	3.24	0.09	2.68

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	23.93	1.20	5.03
2	16	6.42	0.43	6.75
3	16	3.28	0.28	8.58

RECOVERY

The recovery of human PARP14 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:4	83	77-90
	1:8	103	98-112

LINEARITY

To assess the linearity of the assay, cell lysate samples were spiked with high concentrations of human PARP14 and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Cell lysate
1:2	Average% of Expected	88
	Range (%)	84-93
1:4	Average% of Expected	90
	Range (%)	83-98
1:8	Average% of Expected	100
	Range (%)	93-106
1:16	Average% of Expected	114
	Range (%)	104-125

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human PARP14 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)