DESCRIPTION

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	20	10.18	0.48	4.70
2	20	2.51	0.14	5.76
3	20	0.70	0.03	3.93

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	24	10.14	0.50	4.94
2	24	2.50	0.14	5.49
3	24	0.77	0.06	7.53

RECOVERY

The recovery of human SMAD2 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	77	71-81
	1:4	99	94-105
Cell lysate	1:8	105	97-112

LINEARITY

To assess the linearity of the assay, human serum samples were spiked with high concentrations of human SMAD2 and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. Cell lysate samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:2.)

		Human serum(Sample Diluent PT 1-ef)	Cell lysate(Sample Diluent PT 4B1)
1:2	Average% of Expected	82	100
	Range (%)	81-84	-
1:4	Average% of Expected	95	106
	Range (%)	88-102	104-109
1:8	Average% of Expected	99	96
	Range (%)	87-110	92-101
1:16	Average% of Expected	102	115
	Range (%)	83-121	99-130

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human SMAD2 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)