DESCRIPTION

Sodium channel regulatory subunit beta-3 (SCN3B) is a regulatory subunit of the voltage-gated sodium channel complex. It modulates the inactivation kinetics of the sodium channel, which is crucial for the generation and propagation of action potentials in neurons and muscle cells. SCN3B has been shown to play a role in various cellular processes, including cell migration and apoptosis. In breast cancer, SCN3B has been identified as an anti-cancer molecule with migration inhibition effects. Additionally, SCN3B emerges as a promising potential prognostic biomarker for glioma, especially for oligodendroglioma.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,350.7	36.4	1.5
2	8	542.2	12.0	2.2
3	8	291.2	21.8	7.5

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,302.3	66.8	2.9
2	16	541.4	12.4	2.3
3	16	286.8	16.0	5.6

RECOVERY

The recovery of human SCN3B spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:32	88	81-91

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:2. )

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	114
	Range (%)	109-119
1:8	Average% of Expected	107
	Range (%)	99-115
1:16	Average% of Expected	107
	Range (%)	100-115

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human SCN3B Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)