DESCRIPTION

SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood. Alteration of SHBG levels have been linked to risks of insulin resistance, cardiovascular disease, male infertility and other diseases.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with biotin) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with biotin also binds to the analyte. Streptavidin-HRP binds to the biotin. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pmol/L)	SD	CV%
1	20	32.0	2.0	6.3
2	20	116.8	4.3	3.7
3	20	439.6	31.1	7.1

Inter-assay Precision

Sample	n	Mean (pmol/L)	SD	CV%
1	24	28.3	2.8	9.9
2	24	116.9	11.2	9.6
3	24	512.1	49.1	9.6

RECOVERY

The recovery of human SHBG spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:400	113	100-123
	1:800	102	91-111

LINEARITY

To assess the linearity of the assay, human serum samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum
1:100	Average% of Expected	100
	Range (%)	-
1:200	Average% of Expected	103
	Range (%)	99-110
1:400	Average% of Expected	97
	Range (%)	87-115
1:800	Average% of Expected	89
	Range (%)	78-109

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human SHBG produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)