DESCRIPTION

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. S100B is highly expressed in astrocytes in the brain. It is also expressed in the Schwann cells of the peripheral nervous system, and outside the nervous system in melanocytes, adipocytes and chondrocyte. Elevated S100B levels in cerebrospinal fluid and serum reported after head trauma, subarachnoid hemorrhage, and stroke were correlated with the extent of brain damage. An association between elevated serum S100B and the presence of brain metastases has been reported. Increased S100B levels may also be found in patients with cerebral small vessel disease (SVD).

PRINCIPLE OF THE ASSAY

A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody also binds to the analyte. Horseradish peroxidase (HRP)-conjugated secondary antibody binds to the detection antibody. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	87.5	3.4	3.9
2	20	302.7	16.2	5.3
3	20	923.9	49.4	5.3

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	90.3	6.5	7.2
2	24	286.2	13.9	4.9
3	24	867.3	46.2	5.3

RECOVERY

The recovery of human S100B spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:2	77	71-87
	1:4	86	79-98
Cell culture supernatant	1:2	89	84-97
	1:4	95	86-102

LINEARITY

To assess the linearity of the assay, three samples were spiked with high concentrations of human S100B in various matrices and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human plasma	Cell culture supernatant
1:2	Average% of Expected	89	100
	Range (%)	82-96	96-107
1:4	Average% of Expected	98	99
	Range (%)	97-99	94-109
1:8	Average% of Expected	105	99
	Range (%)	104-106	98-101
1:16	Average% of Expected	103	98
	Range (%)	101-106	94-102

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human S100B produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)