DESCRIPTION

RPS3 (Ribosomal Protein S3) is a multifunctional protein that plays a crucial role in protein synthesis as a component of the 40S ribosomal subunit. It is involved in various extraribosomal activities, including DNA repair, apoptosis, and the innate immune response to bacterial infection.RPS3 acts as a DNA repair endonuclease, participating in the repair of damaged DNA. It also plays a role in apoptosis, with its localization changing to the nuclear membrane during RPS3-induced apoptosis in lymphocytic cells. Additionally, RPS3 is involved in transcriptional regulation and has been implicated in processes such as cell proliferation and differentiation.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	11.47	1.02	8.93
2	8	2.81	0.21	7.42
3	8	1.13	0.11	9.41

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	11.45	1.09	9.53
2	16	2.76	0.25	8.97
3	16	1.18	0.12	9.80

RECOVERY

The recovery of human RPS3 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:16	117	112-120

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	85
	Range (%)	85-86
1:8	Average% of Expected	93
	Range (%)	93-94
1:16	Average% of Expected	98
	Range (%)	94-102

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human RPS3 Sandwich ELISA Kit produced at Sunlong Medical™.