DESCRIPTION

RGS12 (Regulator of G-protein Signaling 12) belongs to the superfamily of RGS proteins defined by a conserved RGS domain that canonically binds and deactivates heterotrimeric G-proteins. It negatively regulates G-protein-coupled receptor (GPCR) signaling by accelerating GTP hydrolysis on Gα subunits. Unlike typical RGS proteins, RGS12 uniquely integrates multiple signaling domains, including a PDZ domain, PTB domain, and Ras-binding domain (RBD), enabling it to orchestrate cross-talk between GPCRs, receptor tyrosine kinases (RTKs), and mitogen-activated protein kinase (MAPK) pathways. It plays critical roles in neuronal development, osteoclast differentiation, and immune response by modulating calcium channels, transcription factors, and cell cycle progression.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,211.8	41.2	3.4
2	8	294.9	5.4	1.8
3	8	136.0	5.9	4.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,263.3	75.2	6.0
2	16	282.2	14.0	4.9
3	16	136.5	5.7	4.2

RECOVERY

The recovery of human RGS12 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:4,096	96	91-102

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:256. )

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	102
	Range (%)	84-119
1:8	Average% of Expected	114
	Range (%)	108-120
1:16	Average% of Expected	112
	Range (%)	101-123

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human RGS12 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)