DESCRIPTION

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and is a modulator of adipocyte lipid metabolism. PLIN1 is the major cAMPdependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue and mammary gland. The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that derives in an abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	1,122.3	24.4	2.2
2	20	267.2	7.4	2.8
3	20	63.6	3.3	5.1

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	1,188.8	42.8	3.6
2	24	284.8	10.0	3.5
3	24	71.9	9.1	12.6

RECOVERY

The recovery of human/mouse Perilipin 1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:16	78	77-79
Tissue lysate	1:128	92	89-95

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The tissue lysate was initially diluted 1:8. )

		Cell lysate	Tissue lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	90	104
	Range (%)	90-91	103-106
1:8	Average% of Expected	95	114
	Range (%)	94-96	112-116
1:16	Average% of Expected	100	109
	Range (%)	96-104	101-116

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human/mouse Perilipin 1 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)